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Description
Rat IL-9 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 500pg/mL). Then dilute to the following concentrations: 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.8125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 500pg/mL standard working solution into the first EP tube and mix thoroughly to make a 250pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 9 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-9 (IL-9) is a multipotent cytokine (cell signaling molecule) belonging to the interleukin group. It is produced in varying amounts by various cell types, including mast cells, NKT cells, Th2, Th17, Treg, ILC2, and Th9 cells. Th9 cells are considered the primary CD4+ T cells producing IL-9. This cytokine stimulates cell proliferation and prevents apoptosis. It acts through the interleukin-9 receptor (IL9R), activating various signal transducer and activator (STAT) proteins, namely STAT1, STAT3, and STAT5, thus linking this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.8-500 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 1394 reviews
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Product Reviews
★★★★★ 5
Gentle cleanser for dry and sensitive skin.
Style: Tolerance Daily Foaming Facial Cleanser, Style: Tolerance Daily Foaming Facial Cleanser
I have dry and sensitive skin, and this cleanser has worked very well for me. It is very gentle on the skin and has not caused any irritation.
The texture is light and foamy, it spreads easily across the face and rinses off without leaving any residue. After using it, my skin feels clean, soft, and comfortable, without any tightness.
The size is quite large, which I find great because it lasts a long time with daily use.
Overall, a simple but very pleasant cleanser for sensitive skin.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2026
★★★★★ 5
Miracle cream
Size: 13.5 Ounce (Pack of 1)
I have been having crazy acne due to a severe reaction to salicylic acid, among other things. My face was incredibly inflamed, itchy, sensitive, red and painful. My dermatologist started me on an oral antibiotic and a clindamycin/benzoyl peroxide topical. The topical was still drying me out quite a bit so I got this lotion and it's literally a miracle cream. My skin is hydrated, no itchy or stinging and the redness has gone down so much. It's also helping my open sores to heal more quickly. Yes, it's a combination of all 3 products working together but I can definitely see and feel the cream repairing my skin barrier. I will be starting on accutane after a month of antibiotics and will continue to use this balm to keep me from drying up like a prune.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2026
★★★★★ 5
Works amazing! My eczema is clearing up on my face!
Size: 13.5 Ounce (Pack of 1)
I've been struggling with eczema all of my life and I'm insanely sensitive to all sorts of creams. I've tried just about everything you could find. Colloidal oatmeal ones...to hypo allergenic creams in high end stores. I react to goat's milk and even ones with no fragrance. Well, for the first time ever, I found a cream that seems to be doing the trick. I'm on meds to help with the eczema, but the issue was the eye area, upper forehead [between eyebrows], and right under the nose and around the lips. I'd always get this redness and it didn't matter what creams I used. I recently switched to a Clinique soap [more like a moisturizer that comes out of a bottle] for my face, but it still wasn't getting better. It helped, but the redness wasn't going away!
I read online about this cream. I don't use a ton. I put literally a drop under each eye and gently tap it into the area. I also just dab a tiny amount on any other red spot. I'm primarily using this for areas where the redness won't go away. Well, three days after starting this my skin is FINALLY healing around my eyes and in the other zones. My mom even noticed!
I can tell you, this is a THICK cream. It's too heavy to slather all over your face. Use it to target areas and use a lighter moisturizer [Aveeno has a sensitive face cream that's new and works]. This cream will help the skin heal [all those annoying patches and redness]. It's worth the money. I suspect it's going to take me ages to finish this bottle off. But, it just is so nice to see my face and not have some sort of weird raccoon look.
FYI - I do have some patches on my legs which I glob it on and it's helping that heal too. If you need a good hydrating cream that helps protect the skin and helps it get over that bump where it's irritated, this may work for you. Oh, it has absolutely no scent - none. That was a nice surprise. It's designed for sensitive skin. Initially, I was worried about the cost, but now that my eczema is healing up, it's worth every penny. This worked better than all those steroids and hydrocortisone my docs kept giving me. I was so stressed over the wrinkles under my eyes and for once, it's looking nice. :)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 24, 2023
★★★★★ 5
Amazing!! Redness and itchiness gone after 1 day!
Size: 6.76 Ounce (Pack of 1), Size: 6.76 Ounce (Pack of 1)
So, I've had really bad rashes around my eyes and cheeks for a few years. The doctors can't tell me what it is but just used to prescribe me steroid cream which thinned my skin and gave me eye wrinkles. I read about this in a post on Pinterest and ordered it immediately as I had a recurring rash on my face for 3 weeks that kept coming back. Yes it's $32 but I was DESPERATE. Everything else I tried burned my face, even products with the eczema association seal! I was so excited when this came in the mail. I was tiding myself over with Clinique's Dramatically Different Moisturizing Cream and yes, it hydrated my dry face but it stung on contact a bit, and also made my skin tight and began to peel, not painfully but still gross.
So I finally got a chance to put this xeracalm product on my face last night. I put about a quarter size amount at first, then two extra pea sized amounts under my eyes. Unfortunately through the night my face soaked it all up (my skin is EXTREMELY DRY and it's winter now) but as soon as I woke up and look in the mirror my redness was 90% gone. My face was a little tight but not uncomfortably, and definitely no peeling. It also does not burn on contact which is so great for me.
It's called a balm but it's really more like a heavy thick cream. The top is interesting as you just squeeze it and it pops out and dispenses product to not contaminate it. If you have a problem with the dispenser you have to be an idiot, I just have to be honest lol. It's not rocket science. It serves a very important purpose. Also, I was surprised at the size. $32 seemed pricey but for the amount of product I got it is totally appropriate. I only see myself using this for when my skin gets REALLY DRY and not for regular use though. And a little goes a long way. I'm hoping to see my face go back to normal in a few days. I will definitely update!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 29, 2016
★★★★★ 5
This is SO helpful for Dyshidrotic Dermantitis
Size: 6.76 Ounce (Pack of 1)
I have dyshidrotic dermatitis and this helps me with the dryness and itchy feet. It is pretty greasy but I have to keep socks on so I don't care. It works the best if you use it twice a day but still works well once a day.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
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