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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.8 ★★★★★
Based on 1685 reviews
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Product Reviews
★★★★★ 5
Easy and simple: Exact Step-by-Step Guide to Reloading Your Balance
Reload type: One Time Reload
I found reloading my Amazon gift card balance much easier than expected. The process was simple and took about 5 minutes at most. Here are the exact steps to do it:
Login: In the Amazon app or on Amazon.com, log in to your account.
Menu: Tap the 3 lines on the bottom right corner of your screen (next to Rufus, Amazon's AI).
Find Category: In the section highlighted "Shop by category," tap on Gifting & Registry (3rd option down in that section) and a drop-down menu will appear.
Select Gift Cards: On the drop-down menu, tap Gift Cards (first option).
Choose Your Action: "The Gift Card Shop" will appear. There you will have three options: "Redeem a gift card," "View your balance," and "Reload."
Start Reload: To add funds, tap Reload.
Select Type: Scroll down to "Add funds directly to your Amazon Gift Card balance" and tap which reload type you want (one-time, low balance auto-reload, or scheduled auto-reload).
Enter Amount: Choose your reload amount. The default is $25, but you can type in your own amount as well (the minimum amount is $5.00).
Finish: Tap the orange "Buy Now" tab.
***I used the Amazon app using the u i OneUI version 8.0 these Amazon app on Android version 16 (Baklava) on a Samsung Galaxy S25 Ultra. Others may have a slight variation of the menu setup on the Amazon app.***
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Easy way to add balance
Reload type: One Time Reload
Reloading my Amazon gift card balance has been easy and convenient. I like using it when I want to keep a little money set aside for Amazon orders or make checkout quicker without thinking about which card I want to use. The process is straightforward, the balance shows up fast, and it has been reliable every time I have used it. It is also helpful for budgeting because I can add a set amount and then use that balance for regular purchases. Nothing complicated about it, which is exactly why I like it.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Great for Budgeting – Instant Balance and Easy to Use
Reload type: One Time Reload
I use Amazon’s gift card reload feature to plan my spending. It’s perfect when I know I want to make a purchase but prefer to set the funds aside first.
I love that it’s immediate—no worries about pending charges or accidental spending from my card. It gives me peace of mind knowing that when I’m ready to buy, the balance is just sitting there, waiting.
Pros: easy to use, instant balance update, helps with budgeting and avoiding overspending
Cons: funds are locked into Amazon once reloaded, doesn’t count toward certain account requirements like review eligibility
Overall, it’s a convenient and reliable way to manage spending on Amazon, especially if you like having control over your purchases.
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Reviewed in the United States on May 1, 2026
★★★★★ 5
I did this with a pay in 4. It made it super simple for a higher balance.
Reload type: One Time Reload
I’ve used this to reload my balance a few times, and it’s been simple and reliable. The process is quick, and the funds show up without any delays or issues.
It’s a convenient option when you want to control spending or keep purchases separate without using a card each time. Everything is straightforward and easy to manage.
I haven’t run into any problems with transactions or balance updates. It’s been consistent every time I’ve used it.
Overall, it’s an easy and dependable way to keep funds ready to go without any hassle.
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Reviewed in the United States on May 6, 2026
★★★★★ 4
Great for keeping bank statements clean but random security delays are frustrating
Reload type: One Time Reload
I use the balance reload tool to manage my monthly budget and easily hit credit card sign-up bonuses. It keeps my bank statements totally clean by grouping dozens of tiny e-book and movie rental charges into one single transaction. Most of the time the whole process works flawlessly and the money shows up in my account within five minutes. It is honestly the easiest way to put a hard limit on my family's everyday online shopping.
You just need to watch out for a few annoying system quirks when you load larger amounts of money. Even though it usually works instantly the automated security filter sometimes flags big reloads and freezes the transaction for hours. This random delay is incredibly frustrating if you are trying to add cash fast to catch a lightning deal before it expires. You also have to remember that this balance is strictly non-refundable and cannot be used to buy other gift cards.
Overall this is still a great method for organizing online spending and maximizing your cash-back credit card rewards. It totally fixed how I track household purchases but you definitely have to tolerate the occasional processing delays. My best advice is to load your account a day or two before you actually need to buy a big-ticket item. It works perfectly as a budgeting tool as long as you know the rules and plan for the random security hold.
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Reviewed in the United States on April 14, 2026