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Description
Rat CASP12 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a caspase 12 (CASP12) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of caspase 12 (CASP12) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Caspase 12 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Caspase 12 (CASP12) is a protein encoded by the CASP12 gene. This protein belongs to a family of enzymes known as caspases, which cleave their substrates at the C-terminal aspartate residue. It is closely related to caspase 1 and other members of the caspase family, known as inflammatory caspases, which process and activate inflammatory cytokines such as interleukin 1 and interleukin 18. This gene is polymorphic, producing either the full-length caspase protein (Csp12L) or an inactive truncated form (Csp12S). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 1394 reviews
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Product Reviews
★★★★★ 5
After Wasting Money on Kids Tablets… I got these. *Demo Video with 2 Devices*
Color: Gray, Size: 128 GB, Style: Tab A11+, Color: Gray, Size: 128 GB, Style: Tab A11+
I originally bought this tablet for my younger daughter, and after a full year of daily use, I ended up buying it again — and then a third time. Both of my kids (ages 7 and 14) now use the exact same tablet, just with different setups.
And honestly? It’s been a much better choice than any “kids tablet” we’ve tried in the past.
🧠 Why I chose this over a kids tablet:
I’ve purchased multiple kids-specific tablets before, and they were a waste of money.
They were:
slow to load
limited to preset apps
low storage
restricted controls
frustrating to use overall
With this, I can: 👉 set my own parental controls
👉 allow only what I want
👉 still give my kids a fully functional device
It’s a normal tablet — and that’s exactly why it works better.
👩👧👧 Real-life use (2 kids, same tablet)
Both of my daughters use this daily, but in completely different ways:
7-year-old (1 year of use):
YouTube (including shorts — no buffering issues)
Kids games (Google Play)
School work
Taking photos
14-year-old (new user):
Drawing (Ibis Paint X)
Internet browsing
Occasional games
It handles BOTH without any issues.
⚡ Performance & usability
Fast, smooth loading — no lag for everyday use
Can run multiple apps/tabs easily
No overheating, even with extended use
Strong, stable WiFi connection
Great battery life (lasts hours of use)
Charges from 0–100% in about 2 hours
It feels like using a large phone — everything is familiar and easy to navigate.
🖥️ Display & design
Full 11” screen
Clear, bright graphics
Sleek, slim, lightweight design
Extremely portable
Great for videos, drawing, reading, and general use.
✍️ Input & compatibility
Works well with fingertips, stylus, and S Pen
Access to Google Play Store
Syncs with Samsung account
Can connect with other Samsung devices
📦 Storage & expandability
128GB internal storage
Supports microSD (if needed)
We’ve comfortably had:
25–30 apps/games installed before needing to clear space
📸 Camera
Decent quality
Perfectly fine for kids taking photos/videos
🔌 Small things to know
Comes with USB-C cord, but no wall adapter
You’ll need your own charging block
Final Thoughts
This is not marketed as a kids tablet — and that’s exactly why it works so well for kids.
It’s:
faster
more flexible
more reliable
and something they won’t outgrow quickly
After a full year of use and multiple purchases, I can confidently say:
This has been one of the best tech purchases I’ve made for my kids.
❤️ My 14-year-old is just starting to explore digital art, and immediately began working on her very first piece using this tablet and a stylus.
Watching her sit down and just… create without struggling with the tools was honestly the most impressive part for me. No lag, no weird pressure issues, no frustration — it felt natural to her right away. As a parent, that moment alone made this feel worth it. It turned something new and intimidating into something she was excited about instantly. She spent a total of 19 hours on her drawing.
⭐ I included a video showing how both of my kids use the same tablet differently, along with the different case setups we use. The first tablet is 1 year old, the second tablet is brand new.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
Good one, fair price, dependable, would buy again
Color: Gray, Size: 128 GB, Style: Tab A11+
Very satisfied, great screen, when you turn off the default screen setting, is really fast, Manufacturer tries to load too much of their stuff, got to go thru and shut it down one at a time, Overall I am very satisfied!
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Thin, Light, Very Functional - Fantastic tablet
Color: Gray, Size: 256 GB, Style: Tab A11+, Color: Gray, Size: 256 GB, Style: Tab A11+
First off you only get a tablet, an USB-C to USB-C cable, quickstart instructions and Sumsung care info. That's it. No charger, no nothing else. Even the screen doesn't come with a protector, the tablet itself comes in a fabric/paper like bag.
So advice: Buy a screen protector and/or case for this tablet! It's very thin and light weight!
The tablet itself is fantastic. It plays all the games so far I threw at it, even the graphic intense ones. Compared to some Chinese ripoffs, this shines like a champ. The 256 gigs of space is plenty and 8 gigs of ram help alot! Truly the tablet is great for my use - Playing games and watching videos.
I'm a little upset for the fact that it doesn't come with any charging device, but my phone's charger works fine. The USB-C to USB-C cable may come in handy (but my old PRO12 tablet uses the older usb port).
Overall I'm very pleased with it. The games I played on my Moto G phone play fantastic on the A11+ plus it can play much more. Also I don't stream so I put videos on the tablet. My old tablet I had to convert them to make the compatible. The A11+ and Android 16 shines here. It can play most all my videos without conversion, even 4k HDR videos natively! And the sound it great with four speakers as well!
Only downfall is the warranty. When I registered the tablet says something about the country of origin so I don't have warranty for the tablet.
Just remember to have a charger for it, screen protector and I recommend a case!
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Reviewed in the United States on April 17, 2026
★★★★★ 5
All you need in retirement
Color: Gray, Size: 256 GB, Style: Tab A11+
Great purchase in comparison with Fire and the Tab S. Does everything I need it to do: read books, email, pay bills, chat, etc. The price was right and it's a good solid tablet without too much complications. I have a Samsung Galaxy smartphone so both are complementary to each other. One comment, Samsung does not provide an actual plug, just the cable...not the best customer service act for sure but seems like all of them are doing it now. Otherwise, I'm pleased with it.
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Reviewed in the United States on April 22, 2026
★★★★★ 4
No Fingerprint reader.
Color: Gray, Size: 128 GB, Style: Tab A11+
Nice tablet. I find the video quality to just OK. That may be because I haven't found the optimal settings. The biggest drawback is my fault for sure. The tablet doesn't have a fingerprint reader. All my phones and previous tablets had that. I now know it a higher end feature. But as a casual tablet this is fine.
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Reviewed in the United States on April 24, 2026