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Description
Human ATGL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 20ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with adipose triglyceride lipase (ATGL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of adipose triglyceride lipase (ATGL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human adipose triglyceride lipase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Adipose triglyceride lipase, also known as protease domain-containing protein 2 and ATGL, is an enzyme encoded by the PNPLA2 gene. ATGL catalyzes the first reaction in lipolysis, in which triacylglycerols are hydrolyzed to diacylglycerols. ATGL has a very high substrate specificity for triacylglycerols and contains a catalytic dyad that utilizes a serine-aspartate nucleotide. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell culture supernatant |
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4.0 ★★★★★
Based on 1375 reviews
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Product Reviews
★★★★★ 5
Sturdy, Beautiful, and Functional
Color: Black
I love this MacBook stand. It sits at the perfect height (my neck doesn’t hurt anymore!), it matches the Space Black finish on the MacBook, and is very sturdy. I considered the 12 south instead of this, and returned it for this instead. Better value and better product. Overall, I highly recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 12, 2025
★★★★★ 5
Fantastic Stand
Color: Gray, Color: Gray
Cooling - I purchased this stand for use in college, primarily so I could raise my laptop to the same height as my primary monitor. While my focus in purchasing this was on raising my laptop, the stand also helps cool the laptop. I have been thoroughly surprised i its effectiveness. Many times my friends and I will be working on homework together running programs such as Microsoft Teams and Mathworks Matlab simultaneously. In doing so, I will have my laptop on the stand so I can use my monitor. While my friends identical college-issued laptops are very, very hot due to the intensive tasks they are completing, mine is, at the most, warm to the touch. The only difference is that I am using the stand, and they are not. The slots in the stand, while not overly large, do aid in cooling the laptop significantly.
Build Quality - Prior to purchasing this stand, I saw several reviewers mentioning the finish on the stand, claiming it had an almost gritty quality. After receiving the product, I believe those customers were hoping for a very high level of final finishing. That is not to say that it is not a high quality finish, but if you are expecting it feel like the aluminum lid of a Macbook, it will have a slightly coarser finish than that.
The stand has rubber pads on all points it would contact the laptop with. These pads are mounted very securely to the frame, and I am not concerned at all about them falling off. If I would have to guess, they are made of a type of silicone and have shown no signs of wear after 2 months of daily use. They are also present on the bottom of the stand to protect the surface it is set on.
The stand itself is constructed of 4mm thick aluminum all around. As such, it is very sturdy. I would be absolutely shocked if a laptop broke one of these stands. I have even used it to hold textbooks temporarily, and it has held them with supreme ease.
Color - I purchased the model in the darker gray color. I did not have anything I was trying to match it with. As such, I cannot say whether it will match Apple's Space Gray products, although it looks very reminiscent of it.
For reference, my laptop is an HP Elite Dragonfly with a 13.3 inch screen, and my monitor is a 21.5 inch Acer unit.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 10, 2021
★★★★★ 5
Great minimalist stand
Color: Gray, Color: Gray
Really like the minimalist design of this stand. Quality is top notch with steady material and refined edges.
Had to make minor modifications to prevent laptop touching metal edges when putting on with slight angel tilt.
Overall very satisfied with the product.
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Reviewed in the United States on September 13, 2025
★★★★★ 5
Simple upgrade that actually improves your setup
Color: Black
I didn’t expect much from a laptop stand, but this made a noticeable difference right away. It lifts the laptop to a much better eye level, which helps a lot with posture—especially if you’re working for a few hours at a time.
The build quality is solid. It’s aluminum, has a bit of weight to it, and doesn’t feel cheap. Once it’s on the desk, it stays in place. The rubber grips hold the laptop securely, and I haven’t had any slipping issues.
I’m using it with a MacBook Pro and it fits perfectly. There’s enough space underneath for airflow, and I’ve actually noticed the laptop runs a bit cooler compared to sitting flat on the desk.
Overall, it’s a simple product but very effective. One of those small upgrades that you end up appreciating every day.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
I like it just fine. It’s good and it works for me.
Color: Black
It’s sturdy and I like the design. Seems pretty comparable to every other MacBook stand, but I chose this one for some reason and I’m happy with it. The height is not adjustable, so if you want that find another one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2025
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